期刊
VIROLOGY
卷 462, 期 -, 页码 42-48出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.05.024
关键词
HCV; MicroRNA; Replicon; Polyphenol
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology, Japan [25293076, 25860520, 24390183]
- Health Sciences Research Grants of The Ministry of Health, Labour and Welfare of Japan
- Mishima Kaiun Memorial Foundation
- Honjo International Scholarship Foundation
- Japanese Society of Gastroenterology
- Grants-in-Aid for Scientific Research [24390183, 25293076, 25860520] Funding Source: KAKEN
Despite recent progress in the development of direct-acting antivirals against hepatitis C virus (HCV), chronic HCV infection remains an important health burden worldwide. MicroRNA122 (miR122), a liver-specific microRNA (miRNA), positively regulates HCV replication, and systemic application of antisense oligonucleotides against miR122 led to the long-lasting suppression of HCV viremia in human clinical trials. Here, we report that apigenin, a flavonoid and an inhibitor of maturation of a subset of miRNAs, inhibits HCV replication in vitro. Apigenin decreased the expression levels of mature miR122 without significantly affecting cell growth. Because supplementation of synthesized miR122 oligonucleotides or overexpression of constitutively active TRBP blocked these effects, the inhibitory effects of apigenin on HCV replication seemed to be dependent on the reduction of mature miR122 expression levels through inhibition of TRBP phosphorylation. Thus, apigenin intake, either through regular diet or supplements, may decrease HCV replication in chronically infected patients. (C) 2014 The Authors. Published by Elsevier Inc.
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