期刊
VIROLOGY
卷 466, 期 -, 页码 112-122出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.05.013
关键词
Metagenomics; Marine virology; Tara-Oceans; Phylogenetic mapping; Giant DNA virus
类别
资金
- France Genomique [ANR-10-INBS-0009]
- CNRS
- Aix-Marseille University
Since the pioneering Global Ocean Sampling project, large-scale sequencing of environmental DNA has become a common approach to assess the biodiversity of diverse environments, with an emphasis on microbial populations: unicellular eukaryotes (protists), bacteria, archaea, and their innumerous associated viruses and phages. However, the global analysis of the viral diversity (the virome) from sequence data is fundamentally hampered by the lack of a universal gene that would allow their unambiguous identification and reliable separation from cellular microorganisms. The problem has been made even more difficult with the discovery of micron-sized giant viruses for which the usual fractionation protocol on a sterilizing filter is no longer an option. In the present proof-of-principle work we used actual metagenomic data to show that glutamine-hydrolysing asparagine synthase is a reliable sequence probe to discover new members of the Mimiviridae family, hint at the existence of a new family of large DNA viruses, and point out misidentified database entries. (C) 2014 Elsevier Inc. All rights reserved.
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