期刊
VIROLOGY
卷 462, 期 -, 页码 166-174出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2014.05.017
关键词
Hepatitis C virus; Rab18; Viral assembly; Lipid droplet; Core protein; RNA replication
类别
资金
- Ministry of Health, Labor, and Welfare of Japan
- Japan Society for the Promotion of Science (JSPS) [25293110]
- Grants-in-Aid for Scientific Research [25293110] Funding Source: KAKEN
During persistent infection of HCV, the HCV core protein (HCV-JFH-1 strain of genotype 2a) is recruited to lipid droplets (LDs) for viral assembly, but the mechanism of recruitment of the HCV core protein is uncertain. Here, we demonstrated that one of the Ras-related small GTPases, Rab18, was required for trafficking of the core protein around LDs. The knockdown of Rab18 reduced intracellular and extracellular viral infectivity, but not intracellular viral replication in HCV-JFH-1-infected RSc cells (an HuH-7-derived cell line). Exogenous expression of Rab18 increased extracellular viral infectivity almost two-fold. Furthermore, Rab18 was co-localized with the core protein in HCV-JFH-1-infected RSc cells, and the knockdown of Rab18 blocked recruitment of the HCV-JFH-1 core protein to LDs. These results suggest that Rab18 has an important role in viral assembly through the trafficking of the core protein to LDs. (C) 2014 Elsevier Inc. All rights reserved.
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