期刊
VIROLOGY
卷 438, 期 2, 页码 61-69出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2012.12.012
关键词
Polerovirus; Luteoviridae; Small RNA; sgRNA3; RNA-binding protein; PLRV; ORF7
类别
资金
- ACIDF [2010C001R]
- NSERC
- AAFC Research Affiliate Program
- Australian Endeavor Fellowship
- Research Council Fellowship
Potato leafroll virus (PLRV) is a positive-strand RNA virus that generates subgenomic RNAs (sgRNA) for expression of 3' proximal genes. Small RNA (sRNA) sequencing and mapping of the PLRV-derived sRNAs revealed coverage of the entire viral genome with the exception of four distinctive gaps. Remarkably, these gaps mapped to areas of PLRV genome with extensive secondary structures, such as the internal ribosome entry site and 5' transcriptional start site of sgRNA1 and sgRNA2. The last gap mapped to similar to 500 nt from the 3' terminus of PLRV genome and suggested the possible presence of an additional sgRNA for PLRV. Quantitative real-time PCR and northern blot analysis confirmed the expression of sgRNA3 and subsequent analyses placed its 5' transcriptional start site at position 5347 of PLRV genome. A regulatory role is proposed for the PLRV sgRNA3 as it encodes for an RNA-binding protein with specificity to the 5' of PLRV genomic RNA. Crown Copyright (C) 2013 Published by Elsevier Inc. All rights reserved.
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