期刊
VIROLOGY
卷 438, 期 1, 页码 14-19出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2012.11.020
关键词
Classical swine fever virus; Pestivirus; Virulence; Quasispecies estimation; Haplotype reconstruction; ShoRAH; QuRe; QuasiRecomb
类别
资金
- Swiss Federal Veterinary Office (BVET) [1.10.13]
- Swiss National Science Foundation [CR32I2_127017]
- Swiss National Science Foundation (SNF) [CR32I2_127017] Funding Source: Swiss National Science Foundation (SNF)
Classical swine fever virus (CSFV) is a positive-sense RNA virus with a high degree of genetic variability among isolates. High diversity is also found in virulence, with strains covering the complete spectrum from avirulent to highly virulent. The underlying genetic determinants are far from being understood. Since RNA polymerases of RNA viruses lack any proof-reading activity, different genome variations called haplotypes, occur during replication. A set of haplotypes is referred to as a viral quasispecies. Genetic variability can be a fitness advantage through facilitating of a more effective escape from the host immune response. In order to investigate the correlation of quasispecies composition and virulence in vivo, we analyzed next-generation sequencing data of CSFV isolates of varying virulence. Viral samples from pigs infected with the highly virulent isolates Koslov and Brescia showed higher quasispecies diversity and more nucleotide variability, compared to samples of pigs infected with low and moderately virulent isolates. (C) 2013 Elsevier Inc. All rights reserved.
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