期刊
VIROLOGY
卷 432, 期 2, 页码 452-459出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2012.07.002
关键词
HBV; Enhancer II; Interferon-alpha; Protein kinase C
类别
资金
- Grants-in-Aid for Scientific Research [24590973] Funding Source: KAKEN
HBV has two enhancer (En) regions each of which promotes its own transcription. En II regulates production of pregenomic RNA, a key product of HBV replication, more strongly than En I. Although IFN-alpha has been found to suppress En I activity, its effect on En II activity has not been examined. Here we used luciferase assay to demonstrate that IFN-alpha suppresses En II activity. Analysis with several deletion/mutation constructs identified two major segments, nt 1703-1727 and nt 1746-1770, within the En II sequence as being responsible for the suppressive effects of IFN-alpha. Pre-treatment with protein kinase C (PKC) inhibitors blocked this effect regardless of the expression levels of phospho-STAT1 and Mx upon IFN-alpha stimulation. These results indicate that IFN-alpha suppresses En II activity via the PKC pathway, which may be an alternative suppressive pathway for HBV replication. (136 words). (C) 2012 Elsevier Inc. All rights reserved.
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