期刊
VIROLOGY
卷 415, 期 2, 页码 141-152出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2011.04.008
关键词
Tomato bushy stunt virus; Flock House virus; Replication; Host virus interaction; Phospholipid; Host membrane; Cellular localization; Viral replicase
类别
资金
- National Science Foundation [IOB-0517218]
- NIH-NIAID [5R21AI072170-02]
- Kentucky Tobacco Research and Development Center
- Direct For Biological Sciences [0817790] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems [0817790] Funding Source: National Science Foundation
Replication of plus-strand RNA viruses depends on lipids present in cellular membranes. Recent genome-wide screens have revealed that eight phospholipid biosynthesis genes affected the replication of Tomato bushy stunt virus (TBSV) in yeast model host. To test the importance of phospholipids in TBSV replication, we studied one of the identified genes, namely INO2, which forms a heterodimer with Ino4, and is a transcription activator involved in regulation of phospholipid biosynthesis. Deletion of INO2, or double deletion of INO2/IN04, reduced TBSV replication and inhibited the activity of the viral replicase complex. In addition, the stability of the viral replication protein is decreased as well as the localization pattern of the viral protein changed dramatically in ino2 Delta ino4 Delta, yeast. Over-expression of Opi1, a repressor of Ino2 and phospholipid biosynthesis, also inhibited TBSV RNA accumulation. In contrast, over-expression of Ino2 stimulated TBSV RNA accumulation. We also observed an inhibitory effect on Flock house virus (FHV) replication and the reduced stability of the FHV replication protein in ino2 Delta ino4 Delta yeast. These data are consistent with the important role of phospholipids in RNA virus replication. (C) 2011 Elsevier Inc. All rights reserved.
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