期刊
VIROLOGY
卷 413, 期 2, 页码 205-215出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2011.02.008
关键词
Cell-to-cell movement; Endoplasmic reticulum; Replicase complex; Positive-strand RNA virus; Dianthovirus
类别
资金
- Japan Society for the Promotion of Science [19580046, 22580047]
- Grants-in-Aid for Scientific Research [22580047, 19580046, 22380030] Funding Source: KAKEN
Movement protein (MP) of Red clover necrotic mosaic virus (RCNMV) forms punctate structures on the cortical endoplasmic reticulum (ER) of Nicotiana benthamiana cells, which are associated with viral RNA1 replication (Kaido et al., Virology 395, 232-242. 2009). We investigated the significance of ER-targeting by MP during virus movement from cell to cell, by analyzing the function of a series of MPs with varying length deletions at their C-terminus, either fused or not fused with green fluorescent protein (GFP). The C-terminal 70 amino acids were crucial to ER-localization of MP-GFP and cell-to-cell movement of the recombinant virus encoding it. However, C-terminal deletion did not affect MP functions, such as increasing the size exclusion limit of plasmodesmata, single-stranded RNA binding in vitro, and MP interacting in vivo. We discuss the possible role of this MP region in virus movement from cell to cell. (C) 2011 Elsevier Inc. All rights reserved.
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