期刊
VIROLOGY
卷 409, 期 1, 页码 77-83出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2010.09.025
关键词
phospholipase A2; AAV serotype 2; parvovirus; endosomal escape; lipolytic pore formation
类别
资金
- Deutsche Forschungsgemeinschaft [SFB 670, SPP1230]
- Center for Molecular Medicine Cologne (ZMMK)
The unique region of the VP1 capsid protein of adeno-associated viruses (AAV) in common with autonomously replicating parvoviruses comprises a secreted phospholipase A2 (sPLA2) homology domain. While the sPLA2 domain of Minute Virus of Mice has recently been shown to mediate endosomal escape by lipolytic pore formation, experimental evidence for a similar function in AAV infection is still lacking. Here, we explored the function of the sPLA2 domain of AAV by making use of the serotype 2 mutant (HD)-H-76/AN. The sPLA2 defect in (HD)-H-76/AN, which severely impairs AAV's infectivity, could be complemented in trans by co-infection with wild-type AAV2. Furthermore, co-infection with endosomolytically active, but not with inactive adenoviral variants partially rescued (HD)-H-76/AN, providing the first evidence for a function of this domain in endosomal escape of incoming AAV particles. (C) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据