Deep sequencing of Cotesia vestalis bracovirus reveals the complexity of a polydnavirus genome

Here we completed the whole genome sequence of Cotesia vestalis bracovirus (CvBV) by deep sequencing and compared the genome features of CvBV to those of other polydnaviruses (PDVs). The genome is 540,215 base pairs divided into 35 genomic segments that range from 2.6 to 39.2 kb. Comparison of CvBV with other PDVs shows that more segments are found, including new segments that have no corresponding segments in other phylogenetically related PDVs, which suggests that there might be still more segments not being sequenced in the present known PDVs. We identified eight gene families and five genes in CvBV, including new genes which were first found in PDVs. Strikingly, we identified a putative helicase protein displaying similarity to human Pif1 helicase, which has never been reported for other PDVs. This finding will bring new insights in research of these special viruses. (C) 2011 Elsevier Inc. All rights reserved.