期刊
VIROLOGY
卷 413, 期 2, 页码 253-264出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2011.02.022
关键词
Dengue virus; Flavivirus; Non-structural protein NS1; N-linked glycosylation; Complement
类别
资金
- Midwest Regional Centers for Excellence for Biodefense and Emerging Infectious Disease Research [U54-A1057160]
- National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand
- Mahidol University
- National Institutes of Health
- Divisions of Dermatology and Rheumatology in the Department of Medicine, Washington University School of Medicine
Dengue virus (DENV) NS1 is a versatile non-structural glycoprotein that is secreted as a hexamer, binds to the cell surface of infected and uninfected cells, and has immune evasive functions. DENV NS1 displays two conserved N-linked glycans at N130 and N207. In this study, we examined the role of these two N-linked glycans on NS1 secretion, stability, and function. Because some groups have reported reduced yields of infectious DENV when N130 and N207 are changed, we analyzed glycosylation-deficient NS1 phenotypes using a transgenic expression system. We show that the N-linked glycan at position 130 is required for stabilization of the secreted hexamer whereas the N-linked glycan at residue 207 facilitates secretion and extracellular protein stability. Moreover, NS1 mutants lacking an N-linked glycan at N130 did not interact efficiently with complement components C1s and C4. In summary, our results elucidate the contribution of N-linked glycosylation to the function of DENV NS1. (C) 2011 Elsevier Inc. All rights reserved.
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