期刊
VIROLOGY
卷 413, 期 1, 页码 39-46出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2011.01.033
关键词
HIV-1 replication in naive and memory; T-lymphocytes; Cord and adult blood naive and memory; T -lymphocytes; HIV-1 gene expression; CD4; CXCR4 and CCR5 expression on naive and memory T-lymphocytes
类别
资金
- Arizona Biomedical Research Commission [7002]
Our previous study has shown that HIV-1 replicated at higher levels in neonatal (cord) blood monocytes/macrophages and T-lymphocytes compared with adult blood cells. However, it is not known whether this differential HIV-1 replication also occurs in naive and/or memory T-lymphocytes. We, therefore, compared HIV-1 replication in CD3+ and CD4+ naive (CD45RA(+)) and memory (CD45RO(+))T-lymphocytes isolated from five cord and adult blood donors. We found that HIV-1 replicated at higher levels in both CD3+ and CD4+ CD45RA+ and CD45RO(+) T-Iymphocytes isolated from cord blood compared with adult blood. In addition, there was no difference in the cell surface expression of CD4. CXCR4 and CCR5 on cord blood CD45RA+ and CD45RO(+) T-lymphocytes compared with adult blood cells. Furthermore, we found that there was an increase in HIV-1 gene expression in cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells by using a single-cycle replication competent HIV-1-NL4-3-Env(-)R(+) luciferase amphotropic virus, which measures HIV-1 transcriptional activity independent of CD4 and CXCR4 or CCR5 expression. In summary, HIV-1 replicated at higher levels in cord blood CD45RA(+) and CD45RO(+) T-lymphocytes compared with adult blood cells and this differential replication is influenced at the level of HIV-1 gene expression. (C) 2011 Elsevier Inc. All rights reserved.
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