期刊
VIROLOGY
卷 396, 期 1, 页码 125-134出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.10.003
关键词
Influenza virus; RNA-dependent RNA polymerase; RNA polymerase II; Transcription; Gene expression; Ubiquitylation
类别
资金
- MRC
- Wellcome Trust
- European Commission
- Croucher Foundation
- Medical Research Council [G0700848] Funding Source: researchfish
- MRC [G0700848] Funding Source: UKRI
Influenza viruses induce a host shut off mechanism leading to the general inhibition of host gene expression in infected cells. Here, we report that the large subunit of host RNA polymerase II (Pol II) is degraded in infected cells and propose that this degradation is mediated by the viral RNA polymerase that associates with Pol II. We detect increased ubiquitylation of Pol II in infected cells and upon the expression of the viral RNA polymerase suggesting that the proteasome pathway plays a role in Pol II degradation. Furthermore, we find that expression of the viral RNA polymerase results in the inhibition of Pot II transcription. We propose that Pot II inhibition and degradation in influenza virus infected cells could represent a viral strategy to evade host antiviral defense mechanisms. Our results also Suggest a mechanism for the temporal regulation of viral mRNA synthesis. (C) 2009 Elsevier Inc. All rights reserved.
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