期刊
VIROLOGY
卷 406, 期 1, 页码 21-27出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2010.06.045
关键词
Melon aphid-borne yellows virus; RNA silencing suppressor; P0; Trp 212
类别
资金
- National Key Basic Research Program of China [2006CB101903]
- Natural Science Foundation of Beijing, China [6082006]
- Foundation of State Key Laboratory for Agrobiotechnology [2009 SKLAB05-7]
Melon aphid-borne yellows virus (MABYV) is a newly identified polerovirus occurring in China. Here, we demonstrate that the MABYV encoded P0 (P0(MA)) protein is a strong suppressor of post-transcriptional gene silencing (PTGS) with activity comparable to tobacco etch virus (TEV) HC-Pro. In addition we have shown that the LP F-box motif present at the N-terminus of P0(MA) is required for suppressor activity. Detailed mutational analyses on P0(MA) revealed that changing the conserved Trp 212 with non-ring structured amino acids altered silencing suppressor functions. Ala substitutions at positions 12 and 211 for Phe had no effect on P0 suppression-activity, whereas Arg and Glu substitutions had greatly decreased suppressor activity. Furthermore, substitutions targeting Phe at position 30 also resulted in reduced PO suppression-activity. Altogether, these results suggest that ring structured Trp/Phe residues in P0 have important roles in suppressor activity. (C) 2010 Elsevier Inc. All rights reserved.
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