期刊
VIROLOGY
卷 387, 期 1, 页码 11-15出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.02.038
关键词
Bunyaviridae; Rift Valley fever virus; Cryo-electron microscopy; Single-particle averaging; Inter-capsomer contacts
类别
资金
- W.M. Keck Foundation to the Gulf Coast Consortia through the Keck Center for Virus Imaging
- NIH/NIAID Western Regional Center of Excellence for Biodefense and Emerging Infectious Disease Research [U54 A1057156]
- Welch Foundation
Rift Valley fever virus (RVFV; Bunyaviridae; Phlebovirus) is an emerging human and veterinary pathogen causing acute hepatitis in ruminants and has the potential to cause hemorrhagic fever in humans. We report a three-dimensional reconstruction of RVFV vaccine strain MP-12 (RVFV MP-12) by cryo-electron microcopy using icosahedral symmetry of individual virions. Although the genomic core of RVFV MP-12 is apparently poorly ordered, the glycoproteins on the virus surface are highly symmetric and arranged on a T=12 icosahedral lattice. Our RVFV MP-12 structure allowed clear identification of inter-capsomer contacts and definition of possible glycoprotein arrangements within capsomers. This structure provides a detailed model for phleboviruses, opens new avenues for high-resolution Structural studies of the bunyavirus family, and aids the design of antiviral diagnostics and effective subunit vaccines. (C) 2009 Elsevier Inc. All rights reserved.
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