期刊
VIROLOGY
卷 385, 期 2, 页码 383-391出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.12.029
关键词
Vaccinia; Virus attachment; Virus entry; Reporter virus; Heparin; Bafilomycin
类别
资金
- National institutes of Health [R21-AI53404, A148487, NIH 1 UC1 A1062486]
- National Institute of Allergy and Infectious Diseases [RCE-U54-AI57168]
- state of Pennsylvania
Differing and sometimes conflicting data have been reported regarding several aspects of vaccinia virus (VV) entry. To address this, we used a beta-galactosidase reporter virus to monitor virus entry into multiple cell types under varying conditions. Entry into HeLa, B78H1 and L cells was strongly inhibited by heparin whereas entry into Vero and BSC-1 cells was unaffected. Bafilomycin also exhibited variable and cell-type-specific effects on VV entry. Entry into B78H1 and BSC-1 cells was strongly inhibited by bafilomycin whereas entry into Vero and HeLa cells was only partially inhibited suggesting the co-existence of both pH-dependent and pH-independent VV entry pathways in these cell types. Finally, entry into HeLa, B78H1, L and BSC-1 cells exhibited a lag of 6-9 min whereas this delay was undetectable in Vero cells. Our results suggest that VV exploits Multiple cell attachment and entry pathways allowing it to infect a broad range of cells. (c) 2009 Elsevier Inc. All rights reserved.
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