期刊
VIROLOGY
卷 386, 期 2, 页码 407-416出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2009.01.039
关键词
Apple latent spherical virus vector; Virus-induced gene silencing; Arabidopsis thaliana; Tobacco; Cucurbit; Legume
类别
资金
- Grant-in-Aids for KAKENHI [19380026, 18380196, 20380025]
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Grants-in-Aid for Scientific Research [20380025, 18380196, 19380026] Funding Source: KAKEN
Apple latent spherical virus (ALSV) vectors were evaluated for virus-induced gene silencing (VIGS) of endogenous genes among a broad range of plant species. ALSV vectors carrying partial sequences of a Subunit of magnesium chelatase (SU) and phytoene desaturase (PDS) genes induced highly Uniform knockout phenotypes typical of SU and PDS inhibition on model plants such as tobacco and Arabidopsis tholiana, and economically important Crops Such as tomato, legume, and cucurbit species. The silencing phenotypes persisted throughout plant growth in these plants. In addition, ALSV vectors Could be Successfully used to silence a meristem gene, proliferating cell nuclear antigen and disease resistant N gene in tobacco and RCYI gene in A. thaliana. As ALSV infects most host plants symptomlessly and effectively induces stable VIGS for long periods, the ALSV vector is a valuable tool to determine the functions of interested genes among a broad range of plant species. (C) 2009 Elsevier Inc. All rights reserved.
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