期刊
VIROLOGY
卷 374, 期 1, 页码 128-137出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2007.11.035
关键词
mosquito densonucleosis viruses; non-structural proteins; antibodies; Western analysis; immuno-fluorescent assay
类别
资金
- NIAID NIH HHS [R01 AI047139-02, R01 AI147139, R01 AI047139-04, R01 AI047139-05, R01 AI047139-01A2, R21 AI147139, R01 AI047139-03] Funding Source: Medline
Mosquito densonucleosis viruses synthesize two non-structural proteins, NS1 and NS2. While NS1 has been studied relatively well, little is known about NS2. Antiserum was raised against a peptide near the N-terminus of NS2, and used to conduct Western blot analysis and immunofluorescence assays. Western blots revealed a prominent band near the expected size (41 kDa). Immuno-fluorescence studies of mosquito cells transfected with AeDNV indicate that NS2 has a wider distribution pattern than does NS 1, and the distribution pattern appears to be a function of time post-infection. Nuclear localization of NS2 requires intact C-terminus but does not require additional viral proteins. Mutations ranging from complete NS2 knock-out to a single missense amino acid substitution in NS2 can significantly reduce viral replication and production of viable progeny. (c) 2007 Elsevier Inc. All rights reserved.
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