期刊
VIROLOGY
卷 374, 期 2, 页码 256-260出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.02.032
关键词
West Nile virus; flavivirus; arbovirus; host switching; quasispecies; natural selection
类别
资金
- NIAID NIH HHS [R01 AI067380, R21 AI055609-01, R01 AI067380-01A2, R21 AI055609-02, R21 AI055609, AI055609, AI067380, R01 AI067380-02] Funding Source: Medline
- NIGMS NIH HHS [GM060201, R25 GM060201] Funding Source: Medline
- PHS HHS [U54A17158] Funding Source: Medline
To investigate differential evolutionary rates and selective forces of WNV in hosts and vectors, we measured the genetic diversity that arose during alternating passage in mosquitoes and birds. Within-host genetic diversity was monitored in each of three experimentally passed replicates, and the complete genome sequence of each WNV strain was determined after passage. The intrahost genetic diversity that arose during alternating passage was significantly greater than the diversity generated during chicken-only passage and similar to mosquito-only passage. d(N)/d(S) ratios suggested purifying selection similar to chick-passed virus, but not to mosquito-passed virus. Thus, the abundant genetic variation contributed to WNV populations through infection of mosquitoes and the strong purifying selection contributed by infection of birds may be maintained despite frequent host switching. (c) 2008 Elsevier Inc. All rights reserved.
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