期刊
VIROLOGY
卷 377, 期 1, 页码 49-53出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.04.017
关键词
HIV-1 Vif; APOBEOG; Vif PPLP motif; Vif multimerization
类别
资金
- NIAID NIH HHS [AI068490, R01 AI29193, P30 AI54999, R21 AI068490, R21 AI068490-02, P30 AI054999, R01 AI029193, R01 AI029193-17, P30 AI054999-04] Funding Source: Medline
The HIV-1 virion infectivity factor (Vif) is required during viral replication to inactivate the host cell anti-viral factor, APOBEC3G (A3G). Vif binds A3G and a Cullin5-ElonginBC E3 ubiquitin ligase complex which results in the proteasomal degradation of A3G. The Vif PPLP motif (amino acids 161-164) is essential for normal Vif function because mutations in this motif reduce the infectivity of virions produced in T-cells. In this report, we demonstrate that mutation of the Vif PPLP motif reduces Vif binding to A3G without affecting its interaction with ElonginC and Cullin5. We demonstrate that the failure of the Vif mutant to bind A3G resulted in A3G incorporation into assembling virions with loss of viral infectivity. (c) 2008 Elsevier Inc. All rights reserved.
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