期刊
VIROLOGY
卷 372, 期 1, 页码 176-186出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2007.10.011
关键词
alphavirus; mosquito; evolution; pathogenesis; genetics
类别
资金
- NIAID NIH HHS [R01 AI048807-05, AI 418807, R01 AI048807, AI 57156, U54 AI057156] Funding Source: Medline
- PHS HHS [T01/CCT622892] Funding Source: Medline
We evaluated infection of Aedes taeniorhynchus mosquitoes, vectors of Venezuelan equine encephalitis virus (VEEV), using radiolabeled virus and replicon particles expressing green (GFP) or cherry fluorescent protein (CFP). More epidemic VEEV bound to and infected mosquito midguts compared to an enzootic strain, and a small number of midgut cells was preferentially infected. Chimeric replicons infected midgut cells at rates comparable to those of the structural gene donor. The numbers of midgut cells infected averaged 28, and many infections were initiated in only 1-5 cells. Infection by a mixture of GFP- and CFP-expressing replicons indicated that only about 100 midgut cells were susceptible. Intrathoracic injections yielded similar patterns of replication with both VEEV strains, suggesting that midgut infection is the primary limitation to transmission. These results indicate that the structural proteins determine initial infection of a small number of midgut cells, and that VEEV undergoes population bottlenecks during vector infection. (C) 2007 Elsevier Inc. All rights reserved.
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