4.4 Article

Gene expression of the human Torque Teno Virus isolate P/1C1

期刊

VIROLOGY
卷 381, 期 1, 页码 36-45

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2008.08.017

关键词

Anellovirus; TTV; Transcription mapping

类别

资金

  1. Berlin Graduates Program (Nafoeg)
  2. EU [513928]

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Torque Teno Virus (TTV) has been assigned to the floating genus Anellovirus. TTV ssDNA genomes have a size of 3.6 to 3.8 kb and display up to 30% nucleotide diversity, The pathogenic potential Of TTV is Under investigation. To address a putative link of pathogenicity with the observed sequence variations, the transcription profile of P/1C1 (genogroup 1) isolated from a patient diseased with a non A-G hepatitis was analysed. Four mRNAs were identified, which encoded the seven proteins ORFI, ORF1/1, ORF1/2, ORF2, ORF2/2, ORF3 and ORF4. Expression of the ORF1 protein and its splice variant ORF1/1 in cell culture was detected by an ORF1-specific antiserum. Analysis of N-terminal tagged P/1C1-encoded proteins revealed that ORF1 ORF1/1 and ORF1/2 were localised in the nucleoli, ORF3 and ORF4 resided in the nucleoplasm, ORF2/2 appeared either in the nucleoli Or the whole nucleus while ORF2 was the only protein seen in the cytoplasm. (C) 2008 Elsevier Inc. All rights reserved.

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