期刊
ELIFE
卷 4, 期 -, 页码 -出版社
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.10230
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资金
- Deutsche Forschungsgemeinschaft [DFG-274/2-3/SFB655]
- Human Frontier Science Program [RGP0016/2010]
- Center for Regenerative Therapies Dresden
- DIGS -BB Program
- Agencia Nacional de Promotion Cientifica y Tecnologica [PICT-2014-3469]
- Bundesministerium fur Bildung und Forschung [0316169A]
Axolotls are uniquely able to mobilize neural stem cells to regenerate all missing regions of the spinal cord. How a neural stem cell under homeostasis converts after injury to a highly regenerative cell remains unknown. Here, we show that during regeneration, axolotl neural stem cells repress neurogenic genes and reactivate a transcriptional program similar to embryonic neuroepithelial cells. This dedifferentiation includes the acquisition of rapid cell cycles, the switch from neurogenic to proliferative divisions, and the re-expression of planar cell polarity (PCP) pathway components. We show that PCP induction is essential to reorient mitotic spindles along the anterior-posterior axis of elongation, and orthogonal to the cell apical-basal axis. Disruption of this property results in premature neurogenesis and halts regeneration. Our findings reveal a key role for PCP in coordinating the morphogenesis of spinal cord outgrowth with the switch from a homeostatic to a regenerative stem cell that restores missing tissue.
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