期刊
ELIFE
卷 4, 期 -, 页码 -出版社
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.04489
关键词
kinesin-14; hand-over-hand; single molecule biophysics; chromosome segregation; kinesin regulation; kinetochore transport
类别
资金
- European Research Council under the European Community's Seventh Framework Programme (FP7) / ERC grant [203499]
- Austrian Science Fund FWF [SFB F34-B03]
- Austrian Research Promotion Agency (FFG)
- Vienna Science and Technology Fund (WWTF) project [VRG10-11]
- Human Frontiers Science Program Project [RGP0041/2012]
- Research Platform Quantum Phenomena and Nanoscale Biological Systems (QuNaBioS)
- Boehringer Ingelheim
Motors proteins of the conserved kinesin-14 family have important roles in mitotic spindle organization and chromosome segregation. Previous studies have indicated that kinesin-14 motors are non-processive enzymes, working in the context of multi-motor ensembles that collectively organize microtubule networks. Here we show that the yeast kinesin-14 Kar3 generates processive movement as a heterodimer with the non-motor proteins Cik1 or Vik1. By analyzing the single-molecule properties of engineered motors we demonstrate that the non-catalytic domain has a key role in the motility mechanism by acting as a foothold that allows Kar3 to bias translocation towards the minus end. This mechanism rivals the speed and run length of conventional motors, can support transport of the Ndc80 complex in vitro and is critical for Kar3 function in vivo. Our findings provide an example for a non-conventional translocation mechanism and help to explain how Kar3 can substitute for key functions of Dynein in the yeast nucleus.
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