期刊
VETERINARY PARASITOLOGY
卷 196, 期 3-4, 页码 334-340出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2013.02.022
关键词
Cryptosporidium; Cryptosporidiosis; Diagnosis; C meleagridis; GP60; Chickens; Turkeys; Genotyping; Algeria
资金
- High National Veterinary School (ENSV), Algiers, Algeria
- Centers for Disease Control and Prevention (CDC), Atlanta GA, USA
Only a small number of birds have been identified by molecular techniques as having Cryptosporidium meleagridis, the third most important species for human cryptosporidiosis. In this study, using PCR-RFLP analysis of the small subunit (SSU) rRNA gene, we examined the ileum of 90 dead chickens from 23 farms and 57 dead turkeys from 16 farms in Algeria for Cryptosporidium spp. C meleagridis-positive specimens were subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium infection rates were 34% and 44% in chickens and turkeys, respectively, with all positive turkeys (25) and most positive chickens (26/31) having C meleagridis. All C meleagridis specimens belonged to a new subtype family. The frequent occurrence of C meleagridis in chickens and turkeys illustrates the potential for zoonotic transmission of cryptosporidiosis in Algeria. Published by Elsevier B.V.
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