期刊
VETERINARY PARASITOLOGY
卷 197, 期 3-4, 页码 543-548出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2013.06.001
关键词
Sarcocystis species; Bovine eosinophilic myositis; Cattle; Laser capture microdissection; Molecular identification
资金
- Institute for the Promotion of Innovation by Science and Technology in Flanders (I.W.T Vlaanderen)
It has been suggested that Sarcocystis species are associated with bovine eosinophilic myositis (BEM). To date, parasite identification in this myopathy has been based on morphological techniques. The aim of the present study was to use molecular techniques to identify Sarcocystis species inside lesions of BEM. Histologically, BEM lesions of 97 condemned carcasses were examined for the presence of Sarcocystis species. Intralesional and extralesional cysts were collected using laser capture microdissection and the species was determined with a PCR-based technique based on 185 rDNA. Intralesional sarcocysts or remnants were found in BEM lesions in 28% of the carcasses. The majority (82%) of intralesional Sarcocystis species were found to be S. hominis. However S. cruzi and S. hirsuta were also found, as well as an unidentified species. It can be concluded that Sarcocystis species present in lesions of BEM are not restricted to one species. (C) 2013 Elsevier B.V. All rights reserved.
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