期刊
VETERINARY PARASITOLOGY
卷 191, 期 1-2, 页码 123-127出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2012.08.015
关键词
Cryptosporidium; Genotype; Subtype; Buffalo; Calves; Egypt
资金
- Arab Fund for Economic & Social Development Zamalat Program
- National Natural Science Foundation of China [31110103901, 31229005]
Little is known about the diversity and public health significance of Cryptosporidium species in water buffaloes. In this study, we examined the distribution of Cryptosporidium spp. in water buffalo calves in Egypt. Rectal fecal specimens from 179 calves and 359 adults were screened microscopically for Cryptosporidium oocysts using modified Ziehl-Neelsen stain. Cry ptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by DNA sequence analysis of the small-subunit rRNA gene, and Cryptosporidium parvum was subtyped by sequence analysis of the 60 kDa glycoprotein gene. Cryptosporidium ryanae was found in 10 specimens and C. parvum in 7 specimens, with the former belonging to the newly identified C. ryanae buffalo variant and the latter belonging to the subtypes IldA20G1 (in 5 specimens) and IlaA15G1R1 (in 2 specimens). The prevailing occurrence of C. ryanae and the subtype family lid of C. parvum and the absence of C. bovis and C. andersoni represent some features of Cryptosporidium transmission in water buffaloes in Egypt. Published by Elsevier B.V.
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