期刊
VETERINARY PARASITOLOGY
卷 184, 期 1, 页码 25-36出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2011.08.013
关键词
Annexin; Schistosoma bovis; Plasminogen; Anticoagulant activity; Tegument
资金
- Spanish Ministry of Science and Innovation [AGL2007-60413/GAN]
- Ministry of Science and Innovation
- CSIC-FSE
Annexins belong to an evolutionarily conserved multigene family of proteins expressed throughout the animal and plant kingdoms. Although they are soluble cytosolic proteins that lack signal sequences, they have also been detected in extracellular fluids and have been associated with cell surface membranes, where they could be involved in anti-haemostatic and anti-inflammatory functions. Schistosome annexins have been identified on the parasite's tegument surface and excretory/secretory products, but their functions are still unknown. Here we report the cloning, sequencing, in silico analysis, and functional characterization of a Schistosoma bovis annexin. The predicted protein has typical annexin secondary and tertiary structures. Bioassays with the recombinant protein revealed that the protein is biologically active in vitro, showing fibrinolytic and anticoagulant properties. Finally, the expression of the native protein on the tegument surface of S. bovis schistosomula and adult worms is demonstrated, revealing the possibility of exposure to the host's immune system and thus offering a potential vaccine target for the control of schistosomiasis in ruminants. (C) 2011 Elsevier B.V. All rights reserved.
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