期刊
VETERINARY PARASITOLOGY
卷 190, 期 1-2, 页码 29-35出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2012.06.010
关键词
Bobcat; Cytauxzoon; rRNA; Internal transcribed spacer unit; Genetic variability; Florida puma
资金
- Morris Animal Foundation [DO8FE-003]
- University of Georgia
- Federal Aid to Wildlife Restoration Act
- SCWDS sponsorship from fish and wildlife agency in Alabama
- SCWDS sponsorship from fish and wildlife agency in Arkansas
- SCWDS sponsorship from fish and wildlife agency in Florida
- SCWDS sponsorship from fish and wildlife agency in Georgia
- SCWDS sponsorship from fish and wildlife agency in Kansas
- SCWDS sponsorship from fish and wildlife agency in Kentucky
- SCWDS sponsorship from fish and wildlife agency in Louisiana
- SCWDS sponsorship from fish and wildlife agency in Maryland
- SCWDS sponsorship from fish and wildlife agency in Mississippi
- SCWDS sponsorship from fish and wildlife agency in Missouri
- SCWDS sponsorship from fish and wildlife agency in North Carolina
- SCWDS sponsorship from fish and wildlife agency in Oklahoma
- SCWDS sponsorship from fish and wildlife agency in Puerto Rico
- SCWDS sponsorship from fish and wildlife agency in South Carolina
- SCWDS sponsorship from fish and wildlife agency in Tennessee
- SCWDS sponsorship from fish and wildlife agency in Virginia
- SCWDS sponsorship from fish and wildlife agency in West Virginia
Cytauxzoon felis, a tick-borne protozoan parasite, is the causative agent of cytauxzoonosis in domestic cats in the United States. The natural reservoir for this parasite is the bobcat (Lynx rufus), which typically does not develop clinical signs. Although not likely important reservoirs, C. fells has also been detected in pumas (Puma concolor) in Florida and Louisiana. Recent studies suggest that specific genotypes of C. felis that circulate in domestic cats may be associated with variable clinical outcomes and specific spatial locations. In the current study, we investigated the intraspecific variation of the C. fells internal transcribed spacer (ITS)-1 and ITS-2 rRNA regions from 145 wild felids (139 bobcats and six pumas) from 11 states (Florida, Georgia, Kansas, Kentucky, Louisiana, Missouri, North Carolina, North Dakota, South Carolina, Oklahoma, and Pennsylvania). Unambiguous ITS-1 and ITS-2 data were obtained for 144 and 112 samples, respectively, and both ITS-1 and ITS-2 sequences were obtained for 111 (77%) samples. For the ITS-1 region, sequences from 65 samples collected from wild felids were identical to those previously reported in domestic cats, while the other 79 sequences were unique. C felis from 45 bobcats and one puma had ITS-1 sequences identical to the most common sequence reported from domestic cats. Within the ITS-2 region, sequences from 49 bobcats were identical to those previously reported in domestic cats and 63 sequences were unique (with some occurring in more than one bobcat). The most common ITS-2 sequence from domestic cats was also common in wild felids (31 bobcats and a puma). Samples from three pumas from Florida and two bobcats from Missouri had a 40- or 41-bp insert in the ITS-2 similar to one described previously in a domestic cat from Arkansas. Additionally, a previously undescribed 198- or 199-bp insert was detected in the ITS-2 sequence from four bobcats. Collectively, based on combined ITS-1 and ITS-2 sequences, five different genotypes were detected in the wild felids. Genotype ITSa was the most common genotype (11 bobcats and one puma) and fewer numbers of ITSb, ITSe, ITSg, and ITSi were detected in bobcats. These data indicate that, based on ITS-1 and ITS-2 sequences, numerous C. felis strains may circulate in wild felids. (C) 2012 Elsevier B.V. All rights reserved.
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