期刊
VETERINARY PARASITOLOGY
卷 172, 期 1-2, 页码 160-163出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2010.04.027
关键词
Dirofilaria immitis; Dirofilaria repens; Microfilariae; Dog; Blood; Multiplex PCR
资金
- Programma dell Universita per la Ricerca (PUR)
Dirofilaria immitis and Dirofilaria repens are the most common species of filarial nematodes described in the dogs with increasing spread into new geographical areas The diagnosis of canine dirofilariosis is usually based upon the microscopical detection and identification of circulating microfilariae together with ELISA detection of serum circulating heartworm antigens or antibodies The identification of the parasite species using the traditional approaches sometimes can be difficult and can lead to misdiagnosis especially on samples from areas where both Dirofilaria are present In this paper we report a new molecular method based on single-step multiplex PCR to detect and differentiate simultaneously and unequivocally D immitis and D repens on DNA extracted from canine peripheral blood The amplification was performed using a set of primers designed on a portion of the small subunit ribosomal RNA gene of the mitochondrion (12S rDNA) The single-step multiplex PCR here described ensured high (4 mf/ml) sensitivity and specificity with reduced cost and time saving The multiplex PCR assay represents an additional tool for epidemiological studies and routine disease assessment in areas co-endemic for the two Dirofilaria species (C) 2010 Elsevier B V All rights reserved
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