期刊
VETERINARY PARASITOLOGY
卷 165, 期 3-4, 页码 231-240出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2009.07.019
关键词
Leishmania braziliensis complex; Histopathology; Virulence; Pathogenesis; Inflammatory infiltrates; Megakaryocytes
资金
- FAPEMIG (Minas Gerais, Brazil)
- Sustainable Science Institute (SSI, USA)
The aim of this study was to evaluate the histopathological features in tissues of mice infected by human isolates (I, II, and III) or the reference M2903 strain of Leishmania braziliensis complex. BALB/c and C57Bl/6 mice were infected in the hind footpad with 10(6) stationary-phase promastigotes; of L braziliensis complex. The evolution of lesions was observed for 10 weeks and the animals were then euthanized and liver, spleen and popliteal lymph nodes were collected. Tissues were stained with hematoxylin and eosin and analyzed by immunohistochemistry assay. Increased thickness of infected footpads was observed in all animals, lesions were nodular and non-ulcerated. Mice infected with isolate I presented inflammatory infiltrates consisting predominantly of mononuclear cells in all tissues examined, and also a great number of megakaryocytes, compared with other isolates. Infection with isolate II led to an infected footpad enlargement not seen in other isolates. In addition, mononuclear infiltrates in the liver and hemosiderin in spleen were noted. Conversely. mice infected with either isolate III or M2903 strain only showed an increased number of megakaryocytes in spleen. All tissues examined had detectable amastigote forms of Leishmania by immunohistochemistry in all groups Taking together, our results showed an unforeseen behavior of different isolates of L. braziliensis complex that led to diverse pathological findings. (c) 2009 Elsevier B.V All rights reserved
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