期刊
VETERINARY PARASITOLOGY
卷 161, 期 3-4, 页码 194-200出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2009.01.034
关键词
Trypanosoma sp.; Trypanosoma evansi; Random Amplified Polymorphic DNA (RAPDs); Venezuelan isolates
资金
- FONACIT [G-97000634, G-98003462]
- Decanato de Investigacion y Desarrollo-USB [GID21]
Nine Trypanosoma sp. Venezuelan isolates, initially presumed to be T. evansi, were collected from three different hosts, capybara (Apure state), horse (Apure state) and donkey (Guarico state) and compared by the random amplification polymorphic DNA technique (RAPD). Thirty-one to 46 reproducible fragments were obtained with 12 of the 40 primers that were used. Most of the primers detected molecular profiles with few polymorphisms between the seven horse, capybara and donkey isolates. Quantitative analyses of the RAPD profiles of these isolates revealed a high degree of genetic conservation with similarity coefficients between 85.7% and 98.5%. Ten of the primers generated polymorphic RAPD profiles with two of the three Trypanosoma sp. horse isolates, namely TeAp-N/D1 and TeGu-N/D1. The similarity coefficient between these two isolates and the rest, ranged from 57.9% to 68.4% and the corresponding dendrogram clustered TeAp-N/D1 and Te Gu-N/D1 in a genetically distinct group. (C) 2009 Elsevier B.V. All rights reserved.
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