期刊
VETERINARY PARASITOLOGY
卷 160, 期 1-2, 页码 171-174出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2008.10.078
关键词
Horse; Cyathostomins; Strongylus; Drug resistance; Benzimidazoles; Reverse Line Blot hybridisation
资金
- CNR-SAV project [2/7189/27, 2/6191/26]
Five horse farms located in eastern Slovakia were investigated for the presence of benzimidazole-resistant strongyles by faecal egg count reduction test and egg hatch assay. Coprocultures were prepared for each farm from faecal samples taken pre- and post-treatment and harvested larvae were molecularly examined with a Reverse Line Blot assay. Faecal egg count reduction values ranged from 0 to 52.5% and all farms were positive for benzimidazole-resistant cyathostomins. Seven benzimidazole-resistant cyathostomin species were molecularly identified on farms before and also after treatment. These data demonstrate that resistance to benzimidazoles is well established in cyathostomin populations from horse farms in the Slovak Republic and that the molecular assay was able to determine the species-specific distribution of resistant cyathostomins under field conditions. (C) 2008 Published by Elsevier B.V.
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