期刊
VETERINARY PARASITOLOGY
卷 161, 期 3-4, 页码 330-334出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetpar.2009.01.002
关键词
Neospora caninum; Toxoplasma gondii; White-tailed deer; Odocoileus virginianus; Neospora agglutination test; Indirect fluorescent antibody test; Western blot; Enzyme-linked immunosorbent assay
资金
- University of Wisconsin Oshkosh Faculty Development Program
- Direct For Biological Sciences
- Div Of Biological Infrastructure [0753525] Funding Source: National Science Foundation
The white-tailed deer (Odocoileus virginianus) is considered one of the most important wildlife reservoirs of Neospora caninum and Toxoplasma gondii in the US. Sera from white-tailed deer from Minnesota and Iowa were tested for antibodies to N. caninum by four serologic tests including the indirect fluorescent antibody (IFA) test (cut-off 1:25), Neospora caninum agglutination test (cut-off 1:25), an enzyme-linked immunoabsorbent assay, and Western blot (WB). Sera were also tested for antibodies to T. gondii using the modified agglutination test (cut-off 1:25). Of 62 adult deer from Minnesota antibodies to T. gondii were found in 20 (32.2%), N. caninum in 44 (71%), with dual infections in 18 deer. Of 170 (73 fawns, 9 yearlings, 88 adults) deer from Iowa, T. gondii antibodies were present in 91 (53.5%) with 37.0, 55.6 and 67.0% seropositivity in fawns, yearlings, and adults, respectively. Antibodies to N. caninum were found in 150 of 170 (88.2%) by any of the 3 tests (99 by Western blots, 135 by ELISA, 106 by IFA, and 118 by NAT). Dual infections with T. gondii and N. caninum were detected in 47 deer. Very high (84.9%) seropositivity of N. caninum in fawns suggests high rate of congenital transmission of the parasite. Seropositivity in each test at different titers is discussed. Published by Elsevier B.V.
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