期刊
VETERINARY PARASITOLOGY
卷 165, 期 3-4, 页码 318-322出版社
ELSEVIER
DOI: 10.1016/j.vetpar.2009.07.022
关键词
Babesia gibsoni; PCR; 18S rRNA gene; Dog; American Pit Bull Terrier; Italy
A 4-year-old intact female American Pit Bull Terrier from Italy descendant of an American-born bitch was evaluated for anorexia, lethargy, weakness, and intermittent vomiting. Oil physical examination, the dog was dehydrated, had pale mucous membranes, hunched posture and abdominal pain. A moderate anemia was observed. Splenomegaly and hyperechoic regions suspected as infarcts in the spleen were seen on abdominal ultrasound. Based on the suspicion of splenic torsion, splenectomy was performed. After surgery, the clinical condition deteriorated. A follow-up complete blood count demonstrated severe macrocytic normochromic anemia with evidence of marked regeneration, left shift neutrophilia, monocytosis and marked thrombocytopenia. Blood smear evaluation revealed single to multiple, variable sized (1-3 mu m in diameter), and round to oval to band-like piroplasms within many red blood cells consistent with small form Babesia spp. or Theileria spp. A partial segment of the 18S rRNA gene was amplified and the PCR product was analyzed by direct sequencing. The nucleotide sequence was completely identical to that of Babesia gibsoni present in GenBank (R). This is the first molecular detection and characterization of B. gibsoni infection in a sick dog from Italy. (c) 2009 Elsevier B.V. All rights reserved.
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