期刊
VETERINARY MICROBIOLOGY
卷 174, 期 3-4, 页码 496-503出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2014.09.021
关键词
Andrographolide; Avian pathogenic Escherichia coli; Quorum sensing; Chicken type II pneumocyte
资金
- National Natural Science Foundation of China [31372470]
- Jilin University Fundamental Research Funds
Avian pathogenic Escherichia coli (APEC) induce septicemia in chickens by invading type II pneumocytes to breach the blood air barrier. The virulence of APEC can be regulated by quorum sensing (QS). Andrographolide is a QS inhibitor of Pseudomonas aeruginosa (P. aeruginosa). Therefore, we investigate whether andrographolide inhibits the injury of chicken type II pneumocytes by avian pathogenic E. coli O78 (APEC-O78) by disrupting the bacterial QS system. The results showed that sub-MIC of andrographolide significantly reduced the release of lactate dehydrogenase (LDH), F-actin cytoskeleton polymerization, and the degree of the adherence to chicken type II pneumocytes induced by APEC-O78. Further, we found that andrographolide significantly decreased the autoinducer-2 (AI-2) activity and the expression of virulence factors of APEC-O78. These results suggest that andrographolide reduce the pathogenicity of APEC-O78 in chicken type II pneumocytes by interfering QS and decreasing virulence. These results provide new evidence for colibacillosis prevention methods in chickens. (C) 2014 Elsevier B.V. All rights reserved.
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