期刊
VETERINARY MICROBIOLOGY
卷 164, 期 3-4, 页码 307-314出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2013.02.013
关键词
Microbiome; Bovine; Gastrointestinal tract; 16S RNA; Ruminants; Nelore
资金
- CAPES
- CNPq Brazilian
- Wisconsin Bioenergy Initiative
The gastrointestinal tracts (GIT) of herbivores harbor dense and diverse microbiota that has beneficial interactions with the host, particularly for agriculturally relevant animals like ruminants such as cattle. When assessing ruminant health, microbiological indicators are often derived from the rumen or feces. However, it is probable that ruminal and fecal microbiota do not reflect the microbial communities within the GIT of ruminants. To test this, we investigated the compartments of the GIT from a Brazilian Nelore steer and performed a 16S rRNA pyrosequencing analysis on the collected samples. Our results showed high intra-individual variation, with samples clustering according to their location in the GIT including the forestomach, small intestine, and large intestine. Although sequences related to the phyla Firmicutes and Bacteroidetes predominated all samples, there was a remarkable variation at the family level. Comparisons between the microbiota in the rumen, feces, and other GIT components showed distinct differences in microbial community. This work is the first intensive non-culture based GIT microbiota analysis for any ruminant and provides a framework for understanding how host microbiota impact the health of bovines. (c) 2013 Elsevier B.V. All rights reserved.
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