期刊
VETERINARY MICROBIOLOGY
卷 166, 期 3-4, 页码 375-385出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2013.07.001
关键词
Calf diarrhea; Case-control study; Enteric pathogens; Multiplex PCR detection
资金
- Calf Scouring Fund, USDA Cooperative State Research, Education, and Extension Service [2007-35102-18115]
- National Research Foundation on behalf of the Korean Ministry of Education, Science and Technology [2010-0024447]
- VDL RD Fund
- National Research Foundation of Korea [2010-0024447] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Calf diarrhea is a major economic burden for the US cattle industry. A variety of infectious agents are implicated in calf diarrhea and co-infection of multiple pathogens is not uncommon in diarrheic calves. A case-control study was conducted to assess infectious etiologies associated with calf diarrhea in Midwest cattle farms. A total of 199 and 245 fecal samples were obtained from diarrheic and healthy calves, respectively, from 165 cattle farms. Samples were tested by a panel of multiplex PCR assays for 11 enteric pathogens: bovine rotavirus group A (BRV-A), bovine coronavirus (BCoV), bovine viral diarrhea virus (BVDV), bovine enterovirus (BEV), bovine norovirus (BNoV), Nebovirus, bovine torovirus (BToV) Salmonella spp. (Salmonella), Escherichia colt (E. colt) K99, Clostridium perfringens with beta toxin gene and Cryptosporidium parvum (C parvum). The association between diarrhea and detection of each pathogen was analyzed using a multivariate logistic regression model. More than a half of the fecal samples from the diarrheic calves had multiple pathogens. Statistically, BRV-A, BCoV, BNoV, Nebovirus, Salmonella, E. coli K99(+), and C parvum were significantly associated with calf diarrhea (p < 0.05). Among them, C parvum and BRV-A were considered to be the most common enteric pathogens for calf diarrhea with high detection frequency (33.7% and 27.1%) and strong odds ratio (173 and 79.9). Unexpectedly BNoV (OR = 2.0) and Nebovirus (OR = 16.7) were identified with high frequency in diarrheic calves, suggesting these viruses may have a significant contribution to calf diarrhea. (C) 2013 Elsevier B.V. All rights reserved.
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