期刊
VETERINARY MICROBIOLOGY
卷 167, 期 3-4, 页码 455-458出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2013.08.001
关键词
Ehrlichia mineirensis; Electron microscopy; In vitro culture
资金
- POSTICK ITN (Postgraduate training network for capacity building to control ticks and tick-borne diseases) within the FP7-PEOPLE - ITN programme (EU) [238511]
- [TE 01020118]
- [GACR Z60220518]
Recently, we reported the in vitro isolation and the molecular characterization of a new species of Ehrlichia (Ehrlichia mineirensis) from haemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks. This organism shows an ortholog of Ehrlichia canis major immunogenic protein gp36 with a new structure of tandem repeats. In the present study, we used electron microscopy (high pressure freezing and freeze substitution preparative techniques) to characterize morphologically this new agent growing in IDE8 tick cells. The results showed that E. mineirensis shares ultrastructural features with other members of the genus Ehrlichia (Ehrlichia muris, E. canis and Ehrlichia chaffeensis); typical parasitophorous vacuoles (morulae) contain electron-dense and reticulated Ehrlichiae embedded inside a fibrillar matrix. We observed the characteristic Gram-negative-type cell wall composed of both cytoplasmic and rippled outer membrane. We found organisms undergoing binary fission and rarely altered cells with unusual invagination of the cytoplasmic membrane. (C) 2013 Published by Elsevier B.V.
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