期刊
VETERINARY MICROBIOLOGY
卷 164, 期 1-2, 页码 195-200出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2013.02.006
关键词
Aeromonas spp.; Phylogenetic identification; gyrB; rpoD; Enterotoxin gene; Eel
资金
- Chonbuk National University
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [1101002330]
Seventy Aeromonas strains were identified by phylogenetic analysis using housekeeping genes (gyrB and rpoD) in order to investigate etiological agents for aeromoniasis in farmed eels (Anguilla japonica). The phylogenetic analysis showed that Aeromonas aquariorum (n = 22, 31.4%) was the predominant species among the investigated eel strains, followed by Aeromonas caviae (n = 16, 22.9%), A. veronii (n = 13, 18.6%), A. hydrophila (n = 12, 17.1%), A. jandaei (n = 4, 5.7%), A. media (n = 2, 2.9%), and A. trota (n = 1, 1.4%). The potential virulence of the present strains was estimated by performing PCR assays using the following seven virulence genes: cytotoxic enterotoxin (act), two cytotonic enterotoxins (aft and ast), glycerophospholipid:cholesterol acyltransferase (gcaT), DNase (exu), lipase (lip), and flagellin (fla). The detection rates of act, alt, ast, gcaT, exu, lip, and fla among all 70 strains were 91.4%, 55.7%, 27.1%, 97.1%, 95.7%, 100%, and 98.6%, respectively. In genotyping of enterotoxin genes, act(+)/alt(+)/ast(+), act(+)/alt(+)/ast(-), and act(+)/alt(-)/ast(-) genotypes were prevalent in A. hydrophila (8/12 strains), A. aquariorum (13/22 strains), and A. caviae (14/16 strains), respectively, suggesting a high heterogeneity among Aeromonas species. In this study, A. aquariorum, which has been an unrecorded species in Korea, can be an etiological agent for aeromoniasis of eel. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据