期刊
VETERINARY MICROBIOLOGY
卷 158, 期 3-4, 页码 247-258出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2012.02.016
关键词
H3N2 canine influenza virus; Genetic analysis; Phylogenetic analysis; Mice infection
资金
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PATD)
- Program for New Century Excellent Talents in University [NCET-07-0440]
The newly emerging canine influenza virus (CIV) causes considerable concerns for both veterinary and public health. During 2009-2010, six strains of H3N2 influenza virus were isolated from dogs in Jiangsu Province, China. Sequence and phylogenetic analysis of eight gene segments revealed that the six viruses were most similar to a recent canine-derived subtype H3N2 influenza virus isolated in cats from South Korea, which originated from avian strain. By comparing the deduced amino acid sequences of the hemagglutinin 1 (HA1) and neuraminidase (NA) genes of the six Jiangsu isolates against the most similar avian strains, we found that all isolates had several common mutations at the receptor-binding sites, potential glycosylation sites and cleavage site in HA], and antigenic sites in both the HA1 and NA segments. Significantly, a unique two amino acid insertion in the NA stalk was found. Experimental infection of BALB/c mice revealed that viral RNA could be detected in the major rodent organs, such as brain, heart, spleen, kidney, liver and intestine, as well as the lung. All the sampled organs from infected mice showed significant lesions and viral antigen staining. This study highlights the potential of domesticated animals to become a reservoir for influenza virus and the need for surveillance programs to detect cross-species transmission. (C) 2012 Elsevier B.V. All rights reserved.
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