期刊
VETERINARY MICROBIOLOGY
卷 157, 期 1-2, 页码 237-242出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2011.12.002
关键词
Haemophilus parasuis; Cytolethal distending toxin; Serum resistance; Adherence; Invasion
资金
- Program for New Century Excellent Talents in University [NCET-06-0752]
- Program for Changjiang Scholars and Innovative Research Teams in Chinese Universities [IRT0723]
- Guangdong Natural Science Foundation [5200638]
Cytolethal distending toxin (CDT) is proposed to be an important virulence determinant of many pathogens. Although two cdt gene cluster loci have been identified in Haemophilus parasuis strain SH0165, the characteristics of CDTs associated with pathogenesis remain unclear. In this study, three CDT-deficient mutants, cdt-1, cdt-2 and the double-knockout cdt-1cdt-2 (Delta cdt-1, Delta cdt-2 and Delta cdt-1 Delta cdt-2, respectively), were obtained in the H. parasuis serovar 4 clinical strain SC096 using a natural transformation method. Compared to the wild-type SC096 strain, the Delta cdt-1, Delta cdt-2 and Delta cdt-1 Delta cdt-2 mutants showed subtle growth defects and clearly exhibited an increased sensitivity to the bactericidal action of porcine and rabbit sera. Additionally, these mutants had a significantly reduced ability to adhere to and invade porcine umbilicus vein endothelial cells (PUVEC) and porcine kidney epithelial cells (PK-15). These findings suggest that both CDTs in the H. parasuis SC096 strain are involved in serum resistance and adherence and invasion of host cells. (C) 2011 Elsevier B.V. All rights reserved.
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