期刊
VETERINARY MICROBIOLOGY
卷 152, 期 1-2, 页码 161-164出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2011.04.027
关键词
Malasseziosis; Antifungal; Azole cross-resistance; Microdilution
资金
- CNPq
- LAPEMI, Brazil
- National Council for Scientific and Technological Development of Brazil (CNPq)
- Mycology Research Laboratory (Laboratorio de Pesquisas Micologicas/LAPEMI, Universidade Federal de Santa Maria/UFSM, Rio Grande do Sul, Brazil)
Objectives: The first aim of this study was to evaluate the in vitro efficacies of fluconazole, ketoconazole, itraconazole and voriconazole on M. pachydermatis growth inhibition. This study also evaluated M. pachydermatis azole cross-resistance, comparing wild clinical isolates and the same isolates with in vitro-induced fluconazole resistance. Methods: Two techniques were used: (1) a broth microdilution method based on protocol M27-A3 from the Clinical and Laboratory Standards Institute to determine the minimum inhibitory concentration (MIC) and (2) the Fekete-Forgacs method to induce fluconazole resistance in vitro. The isolates were divided into two groups: group 1 included fluconazole-susceptible clinical isolates (n = 30) and group 2 contained the same isolates with in vitro-induced fluconazole resistance (n = 30). Results: The two groups exhibited differences in susceptibility (p < 0.001). Group 1 isolates were susceptible to azoles: ketoconazole (MIC 0.01-1.0 mu g/mL), itraconazole (MIC 0.01-1.0 mu g/mL), voriconazole (MIC 0.01-4.0 mu g/mL), and fluconazole (MIC 0.01-4.0 mu g/mL). Group 2 isolates demonstrated a wider range of MICs to azoles: ITZ (MIC 0.06-64.0 mu g/mL), KTZ (MIC 0.25-32.0 mu g/mL), VRZ (MIC 2.0-128.0 mu g/mL). and FLZ(MIC 64.0-128.0 mu g/mL). Conclusions: It was shown that FLZ-resistant M. pachydermatis isolates exhibit cross-resistance to other azoles, reinforcing the importance of susceptibility tests as a guide for the therapeutic prescription of antifungals in medical and veterinary mycology. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据