期刊
VETERINARY MICROBIOLOGY
卷 148, 期 2-4, 页码 189-199出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2010.09.013
关键词
Foot-and-mouth disease virus; Conformational epitope; Protective monoclonal antibody; Phage display; Reverse genetic system
资金
- National High Technology Research and Development Program of China [2006AA10A203]
- Heilongjiang Provincial Science and Technology Program [GA06B202]
Although neutralizing antigenic sites of foot-and-mouth disease virus (FMDV) can be defined by selection of monoclonal antibody (MAb) escape mutants, no conformational neutralizing epitope on the major antigenic site located on the G-H loop of type Asia1 FMDV has been precisely mapped. In this study, we generated a potent neutralizing MAb 3E11, which recognized a conformation-dependent epitope and neutralized FMDV Asia1/YS/CHA/05 in vitro. Importantly, a dose of 5.5 NT50 of the MAb 3E11 completely protected suckling mice from a dose of 10 LD50 of homologous virus challenge in vivo. Through a 12-mer random peptide phage display, synthetic peptide analysis and constructing a series of FMDV Asia1/YS/CHA/05 mutants using reverse genetic system, we finely mapped the neutralizing epitope as the 12-amino acid peptide (141)SXRGXLXXLXRR(152). These results provide additional insights into the virus-MAb interaction at the amino acid level and may help in the development of an epitope-based Asia1 FMDV vaccine. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.
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