期刊
VETERINARY MICROBIOLOGY
卷 145, 期 3-4, 页码 299-307出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2010.04.004
关键词
Diarrhoea; Post-weaning; Antimicrobial; Multi-drug resistance; Virulence
资金
- Pig Health and Research Unit, Bendigo, Victoria
- Department of Primary Industries, Toowoomba, Queensland
- University of South Australia, Adelaide, South Australia
- Department of Agriculture, South Perth, Western Australia
- Elizabeth MacArthur Agricultural Institute (EMAI), Menangle, NSW
- Australian Pork Limited [2013]
This study aimed to characterize antimicrobial resistance and virulence genes in multidrug resistant enterotoxigenic Escherichia coli (ETEC) isolates (n = 117) collected from porcine post-weaning diarrhoea cases in Australia (1999-2005). Isolates were serotyped, antibiogram-phenotyped for 12 antimicrobial agents and genotyped by PCR for 30 plasmid-mediated antimicrobial resistance genes (ARGs), 22 intestinal and 38 extra-intestinal E. coli virulence genes (VGs). Nine serogroups were identified, the most prevalent being O149 (46.2%), O141 (11.2%) and Ont (31.6%). None of the isolates showed resistance to ceftiofur or enrofloxacin and 9.4% were resistant to florfenicol. No corresponding extended-spectrum/AmpC beta-lactamase, fluoroquinolone or floR ARGs were detected. An antimicrobial resistance index (ARI) was calculated from the combined data with a weighting for each antimicrobial agent dependent upon its significance to human health. Serogroup 0141 isolates had a significantly higher ARI due to an elevated prevalence of aminoglycoside ARGs and possession of more virulence genes (VGs), including ExPEC or EHEC adhesins (bmaE sfa/focDE, fimH, ihA) in toxin-producing strains that lacked the normally associated F4 and F18 fimbriae. Few associations between ARGs and VGs were apparent, apart from tetC, sfa/focDE and ompT which, for a sub-set of 0141 isolates, suggest possible plasmid acquisition from ExPEC. The multi-drug resistant ETEC ARG/VG profiles indicate a high probability of considerable strain and plasmid diversity, reflecting various selection pressures at the individual farm level rather than emergence and lateral spread of MDR resistant/virulent clones. (C) 2010 Published by Elsevier B.V.
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