期刊
VETERINARY MICROBIOLOGY
卷 143, 期 2-4, 页码 371-378出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2009.11.006
关键词
Porcine reproductive and respiratory syndrome virus (PRRSV); Receptor; Sialoadhesin; Binding domain
资金
- National Basic Research Program of China [2005CB523200]
- National Scientific Supporting Program [2006BAD06A18/04/03/01, 2007BAD86805/03]
- NSFC [30901080]
- National Postdoctoral Foundation [20090451026]
- Heilongjiang Province Postdoctoral Foundation [LBH-Z08015]
Sialoadhesin (Sn) is an important receptor for viral attachment and internalization of porcine reproductive and respiratory syndrome virus (PRRSV) to porcine alveolar macrophages (PAM). To investigate whether the NI-terminal domain of Sn is sufficient and/or necessary for PRRSV attachment, we constructed a series of truncated fragments of porcine Sn and expressed these in the non-permissive PK15 cell line. The first 150 amino acids comprising the entire first domain of the Sn N-terminal region was necessary for PRRSV binding, to cells, and the N-terminal domain alone was sufficient for virus attachment. The attachment of PRRSV to PAM cells was inhibited by polyclonal anti-serum against the N-terminal region of porcine Sn in a dose-dependent manner. The present study demonstrates that the first domain at the N-terminus of Sn mediates PRRSV attachment to PAM cells and contributes to better understanding the interaction between PRRSV and its host cells. (C) 2009 Elsevier B.V. All rights reserved.
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