期刊
VETERINARY MICROBIOLOGY
卷 144, 期 3-4, 页码 310-318出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2010.02.033
关键词
BTV-23; Mononuclear leukocytes; Apoptosis; Immuno-suppression; Interferon-alpha and Caspase-3
资金
- CADRAD, IVRI, India
- ICAR, New Delhi, India
The role of apoptosis in pathogenesis of bluetongue (BT) has been suggested from various in vitro studies. However, to date, no clear data are available regarding BTV-induced apoptosis and its consequences in natural host, sheep. In the present study, bluetongue virus (BTV)-induced apoptosis was studied in sheep blood and splenic mononuclear cells by analyzing annexin(+) propidium iodide(-) early apoptotic cells, DNA ladder pattern, and caspase-3 gene expression. The onset of apoptosis and lymphocyte depletion in viraemic phase and IFN-alpha response indicated the involvement of BTV and IFN-alpha in the pathogenesis of BT. The development of Pasteurella pneumonia in 4 of 7 infected sheep during the experiment pointed to possible BTV-induced immuno-suppression and predisposition to secondary microbial infections. These results have significant implications not only in understanding immuno-pathological consequences but also in studying interactions of BTV with host cells. (C) 2010 Elsevier B.V. All rights reserved.
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