期刊
VETERINARY MICROBIOLOGY
卷 146, 期 1-2, 页码 155-160出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2010.05.006
关键词
Newcastle disease virus; APMV-1; Antarctic; Serology; Real-time PCR
资金
- National Counsel of Technological and Scientific Development (CNPq) [141981/2006-7]
- State of Sao Paulo Research Foundation (FAPESP)
Here we report the isolation of Newcastle disease virus (NDV) from cloacal swabs obtained from penguins in the South Atlantic Antarctic region (62 degrees 08S, 58 degrees 25W). Samples of 100 penguins from King George Island were tested by real-time PCR, of which 2 (2%) were positive for NDV. The positive samples were isolated in embryonated chicken eggs and their matrix and fusion proteins genes were partially sequenced. This was complemented by the serological study performed on the blood of the same specimens, which resulted in a 33.3% rate of positivity. (C) 2010 Elsevier B.V. All rights reserved.
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