期刊
VETERINARY MICROBIOLOGY
卷 142, 期 3-4, 页码 254-260出版社
ELSEVIER
DOI: 10.1016/j.vetmic.2009.10.016
关键词
Neutralization; Cross-neutralization; BoHV-1; BoHV-5
资金
- CNPq
- FAPERGS
- CAPES
- State Government of Rio Grande do Sul
This study was carried out to determine whether the sensitivity of serum neutralization (SN) tests would be affected by the use of distinct subtypes of bovine herpesvirus 1 (BoHV-1) and 5 (BoHV-5) as test challenge viruses. Bovine sera collected from a randomized sample (n = 287) were tested in a 24 h incubation SN against three type 1 viruses (BoHV-1.1 strains Los Angeles (LA) and EVI 123; BoHV-1.2a strain SV 265) and three type 5 viruses (BoHV-5a strain EVI 88; BoHV-5b strain A 663 and BoHV-5c ISO 97). SN sensitivity varied greatly depending on the test challenge virus used in the test, particularly when results against each virus were considered individually, where it ranged from 77% (detecting 80 out of 104 antibody-positive sera) with 150 97 to 91% (95/104) with BoHV-1.1 strain LA. All tests to single viruses revealed a significantly low sensitivity (McNemar's; p < 0.05). Maximum sensitivity (104/104) was achieved when positive results to a particular combination of four of the challenge viruses (LA + EVI 123 + SV 265 + A 663) or some combinations of five viruses (or all six viruses) were added cumulatively. These results provide evidence for no association between any particular virus type/subtype and higher SN sensitivity. In addition, it was clearly shown that when SN is performed with single test challenge viruses, sensitivity can vary so significantly that might compromise control or eradication efforts. Performing SN against a number of different viruses demonstrated to improve significantly the test's sensitivity. (C) 2009 Elsevier B.V. All rights reserved.
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