期刊
VETERINARY MICROBIOLOGY
卷 127, 期 1-2, 页码 50-62出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.vetmic.2007.08.013
关键词
aerosol; air sampling; airborne transmission; classical swine fever virus; quantification
During epidemics of classical swine fever (CSF), neighbourhood infections occurred where none of the 'traditional' routes of transmission like direct animal contact, swill feeding, transport contact or transmission by people could be identified. A hypothesized route of virus introduction for these herds was airborne transmission. In order to better understand this possible transmission route, we developed a method to detect and quantify classical swine fever virus (CSFV) in air samples using gelatine filters. The air samples were collected from CSFV-infected pigs after experimental aerosolization of the virus. Furthermore, we studied the viability of the virus with time in aerosolized state. Three strains of CSFV were aerosolized in an empty isolator and air samples were taken at different time intervals. The virus remained infective in aerosolized state for at least 30 min with half-life time values ranging from 4.5 to 15 min. During animal experiments, concentrations of 10(0.3)-10(1.6) TCID50/ m(3) CSFV were detected in air samples originating from the air of the pig cages and 10(0.4)-10(4.0) TCID50/m(3) from the expired air of infected animals. This is the first study describing the isolation and quantification of CSFV from air samples originating from infected pigs and their cages, supporting previous findings that airborne transmission of CSF is feasible. (c) 2007 Elsevier B.V. All rights reserved.
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